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For the last two weeks I have been helping Jesus Rubio with his research on nutrition on Tilapia. The aim is too see what diet had a better influence on the fish growth. To do that we have to analyze the protein, lipid, fatty acid, ash, humidity concentration in the fish food and in the actual fish.
i mainly worked with protein and lipid concentration. For the determination of the proteins we use the Kjeldahl method, which is a well known method. ...
Folch Method to quantify lipids
Prepare a mixture of chloroform-metano (2:1 v/v) cotaining 0.01% of BHT. Once the solvent has evaporate with a current of nitrogen, the lipids are weighed and the stored in an atmosphere of nitrogen and dissolved in chloroform to prent oxidation.
3 tanks out of 8 needed to be emptied and have all the fish transported to the engorde stage (growth). There was about 700-800 fish in total, from12 to 14 cms. They had to be caught with nets, placed in a 10 lt bucket with formaldehyde and then placed in a 500 lt container on a pickup, heading to the engorde stage (only a 1-2 miunte drive). These tanks had to be cleaned from thw inside with water ...
Seeing that I had work for the weekend, Desi, who was leaving with Ruben and Antonio (Spanish) for a wedding, let me stay at here place with Jesus, fellow Tilapia researcher and avid apuaponics master. After work on friday, I helped Jesus install an aquaponics system he created. He had a 90lt plastic tub with 10 small tilapia, an 8lt bio filter, a small and a large trey and a water pump. You pump ...
Ruben and Antonio are away, so in Mesocosmo its just me and Desi.
2 of my Winning tanks are being treating for a paraiste, 1 other tank is developing the same parasite and another tank might be developing it. These tanks must be kept extrmeley clean, and the feeding must be done slowly to avoid any excess food to sit at the bottom and rot.
I was trained ...